Analysis Note

Positive ControlHeLa cells, Human skeletal muscle tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3 g/ml)

General description

Anti-FADS1, mouse monoclonal, clone 2D9, recognizes the ~50-55 kDa FADS1 n HeLa cells and human skeletal muscle tissue. It is validated for use in ELISA, WB, and IHC (paraffin sections).

Recognizes the ~50-55 kDa FADS1 protein in HeLa cells and human skeletal muscle tissue.

Purified mouse monoclonal antibody. Recognizes the ~50-55 kDa FADS1 protein.

Immunogen

A recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)